Fig. 5

Dispersed cell cultures derived from hEBs.

hEBs were grown for 5 d in suspension and then were dissociated with trypsin and plated as a monolayer on cover slides coated with fibronectin. Shown are cells with fibroblast (A) or neuronal (B) appearance. Insert in (B) is a higher magnification of neurotic outgrowth. The cells with neuritic outgrowth express neurofilament 68Kd as evident by a fluorogenic product in the cells after in situ hybridization with the neuronal marker (C and D). Scale bar, 100 µm.