Table 1 Response of the transfected human HIV-1-CAT and control plasmids in ex vivo T cells and control cell lines
DNA Clone | Cell Type | Stimulus | CAT Activity (% acetylation) | Inducibility | No. of Expts. |
|---|---|---|---|---|---|
CMV-CAT | Jurkat | CM | 78.1 (±9.6) |  | 3 |
(transfection control) |  | PHA/PMA | 90.1 (85.0/95.2) | × 1.15 | 2 |
U937 | CM | 30.7 (±13.0) |  | 4 | |
|  |  | PHA/PMA | 56.2 (±8.5) | × 1.83 | 3 |
| Â | PB T cells | CM | 6.2 (5.5/7.0) | Â | 2 |
|  |  | PHA/PMA | 6.8 (5.6/8.0) | × 1.10 | 2 |
IL-2-CAT | Jurkat | CM | 0.6 (±0.4) |  | 3 |
(T-cell-specific control) |  | PHA/PMA | 43.0 (±5.9) | × 71.67 | 3 |
U937 | CM | 0.6 (0.9/0.3) | Â | 2 | |
|  |  | PHA/PMA | 0.6 (0.8/0.4) | × 1.00 | 2 |
|  | PB T cells | CM | 0.1 (0.0−0.2) |  | 3 |
|  |  | PHA/PMA | 3.9 (±1.1) | × 39.00 | 3 |
pUC-BENN-CAT | Jurkat | CM | 6.3 (±3.7) |  | 4 |
(HIV-1 LTR) |  | PHA/PMA | 16.9 (±6.7) | × 2.68 | 4 |
|  | U937 | CM | 0.8 (±0.4) |  | 3 |
|  |  | PHA/PMA | 0.7 (±0.5) | × 0.87 | 3 |
|  | PB T cells | CM | 0.7 (±0.2) |  | 4 |
|  |  | PHA/PMA | 3.7 (±0.9) | × 5.29 | 3 |
5X RATS-CAT | Jurkat | CM | 0* | Â | 3 |
|  |  | PHA/PMA | 0* | — | 3 |
| Â | U937 | CM | 0* | Â | 3 |
|  |  | PHA/PMA | 0* | — | 3 |
|  | PB T cells | CM | 0.4 (±0.2) |  | 7 |
|  |  | PHA/PMA | 5.1 (±1.8) | × 12.75 | 5 |