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Fig. 1 | Molecular Medicine

Fig. 1

From: Induction of Placental Heme Oxygenase-1 Is Protective Against TNFα-induced Cytotoxicity and Promotes Vessel Relaxation

Fig. 1

Amplification of inducible heme oxygenase (HO-1) and constitutive heme oxygenase (HO-2) mRNA by reverse transcription polymerase chain reaction (RT-PCR) in human placenta, fetal membranes and first-trimester human trophoblast cells. A 625-bp fragment corresponding to HO-1 and a 828-bp fragment corresponding to HO-2 were amplified from 5 µg of total RNA. (A) Term placenta (1); first trimester tissue (2); amnion (3) and chorio-decidua (4). (B) First trimester human trophoblast cells (Troph). Total RNA from rat spleen (RS) and brain (RB) served as positive control for HO-1 and HO-2, respectively. To ensure that RNA was free of genomic DNA, total RNA was treated with RNase-free DNase1. M, 100-bp ladder.

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Molecular Medicine

ISSN: 1528-3658