Fig. 1

Quantification of apolipoprotein E (apoE) and cholesterol in brain and of amyloid-β (Aβ) peptides in plasma and brain. Concentrations for tg2576 (solid line) mice and control (hyphened line) mice are shown at different ages. (A) The levels of apoE were already elevated in the tg2576 mice at 2 months of age, relative to the controls. This elevation remained constant up to 12 months of age and significantly thereafter (*, p < 0.05). (B) The brain total cholesterol levels, isolated and quantified by high performance liquid chromatography (HPLC) were essentially the same in both the tg2576 and control mice at all ages. (C) The levels of plasma Aβ peptides detected by antibody R163 (AβN-40) and antibody R165 (AβN-42). (D) The levels of plasma Aβ peptides, quantified by antibody 6E10 (Aβ1-40/42). In both instances (C and D), the total plasma Aβ was significantly increased (*, p < 0.05) in the tg2576 mice at the ages of 9 and 12 months, relative to controls. (E) and (F) The amounts of water-insoluble and water-soluble Aβ peptides, respectively, in the tg2576 and control mice brains. After 12 months of age, the quantities of total Aβ in the tg2576 mice brain increased in a quasi-exponential mode.