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Table 2 Gel filtration of whole human serum on Sephacryl

From: Human Serum Amyloid P Component is a Single Uncomplexed Pentamer in Whole Serum

Medium

Protein

Ve(ml)

Oligomerization State

Sephacryl S-300HR

SAP

255

decamer

Sephacryl S-300HR

CRP

285

pentamer

Sephacryl S-300

SAP

184

decamer

Sephacryl S-300

CRP

212

pentamer

  1. Serum was loaded without dilution and eluted with TE buffer. Identical results were obtained at pH 7.4 and pH 8.0. SAP, serum amyloid P component; CRP, C-reactive protein; Ve, elution volume.
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Molecular Medicine

ISSN: 1528-3658