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Table 1 Measurement of the effect of multiple peptides of Fas, sFas, and the apoptogen MoAb anti-Fas (CH11) on Fas positive (Jurkat and WC8) and negative (WR19L) cell lines, as variation of the cytoplasmic free Ca2+.

From: VEINCTR-N, an Immunogenic Epitope of Fas (CD95/Apo-I), and Soluble Fas Enhance T-cell Apoptosis in vitro. II. Functional Analysis and Possible Implications in HIV-1 Disease

  

Jurkat

 

WC8

WR19L

 

Positive

 

Positive

 

Positive

 
 

Cells

Fluorescence

Cells

Fluorescence

Cells

Fluorescence

 

(%)

(M)

(%)

(M)

(%)

(M)

Untreated cells

2.7

4.1

4.1

3.7

3.6

3.9

Cells + pept. #1

7.7

5.6

5.3

4.6

3.8

3.7

Cells + VEINCTR-N

29.1

12.7

25.6

10.3

5.3

4.1

Cells + pept. #2

12.5

8.3

10.2

7.9

4.7

2.9

Cells + sFas

32.6

12.3

26.1

10.4

5.8

4.0

Cells + CH11

29.9

11.8

23.2

11.2

3.9

3.7

  1. The analysis was completed by flow cytometry 30 min after staining the pretreated cells with fluo-3, a fluorescent indicator of Ca2+. Data are presented as both percentage of fluo-3 positive cells and mean of fluorescence intensity (M) in each cell preparation. M, mean of fluorescence intensity; sFas, soluble Fas.