Fig. 7

Dual-parameter flow cytograms of FITC-labeled Annexin-V versus PI staining in L. donovani promastigotes. Cells were incubated with media, DMSO (0.2%), 12.5 µM luteolin and 45.5 µM quercetin at 22°C. Normal non-apoptotic cells were negative for both fluorescein isothiocyanate (FITC) and propidium iodide (PI) (lower left panels); relatively early apoptotic cells were labeled by Annexin-V and were negative for PI (lower right panels); late apoptotic, as well as secondary necrotic, cells were labeled both for Annexin-V and PI (upper right panels). DMSO dimethysulfoxide.