Fig. 4

Effect of PSEN1 mutations on the stability of β-catenin.

(A) 10 µg of cystolic-free fraction (CFF) proteins of nonstimulated (0 min) and LiCl-stimulated (60 min) stable transfected wild type (wt) and mutant (mt; I143T, G384A and T113-114ins) presenilin (PSEN1) (HEK) 293 cells and nontransfected HEK 293 cells (293), were separated on a 10% sodium dodecyl sulphate (SDS) gel and evaluated for β-catenin expression. (B) Plot of normalized cytosolic-free β-catenin values without LiCl stimulation of five independent experiments. Symbols: data points are plotted using black dots; the black horizontal bar (—) indicates the median of each set of data. (C) 10 µg of total brain extract of I143T PSEN1 Alzheimer disease (AD) patients, G384A PSEN1 AD patients, sporadic AD patients, and controls were separated on a 10% SDS gel and immunoblotted with anti-β-catenin.