Fig. 1From: CD39 Modulates Endothelial Cell Activation and Apoptosis CD39 decreases ATP-induced von Willebrand (vWF) release. HUVEC were grown to confluency in 24-well plates, left untreated or infected with CD39 or β-galactosidase adenovirus. Confluent cells were incubated with thrombin, ATP, ATPγS, or adenosine (ADO). vWF release was measured in conditioned serum-free medium, taken 60 min after stimulation by ELISA. Overexpression of CD39 limited ATP-induced vWF secretion by approximately 60%. Experiments were performed in triplicate and results are expressed as mean ± SEM. C, control; NI, non-infected; rAdCD39, recombinant replication-deficient adenovirus from human CD39 cDNA; rAdβgal, recombinant replication-deficient adenovirus from bacterial β-galactosidase.Back to article page