Fig. 2
From: CD39 Modulates Endothelial Cell Activation and Apoptosis
CD39 inhibits ATP-induced E-selectin surface expression.
Confluent human umbilical vein endothelial cells (HUVEC) were left untreated or infected with CD39 or β-galactosidase adenovirus. Cells were then stimulated with thrombin, ATPγS, ATP, or adenosine for 12 hr. E-selectin surface expression was determined by ELISA, in triplicates of the cultures. Overexpression of CD39 approximately halved ATP-induced E-selectin surface expression. Results are expressed as mean ± SEM of an experiment representative of three. C, control; NI, non-infected; rAdCD39, recombinant replication-deficient adenovirus from human CD39 cDNA; rAdβgal, recombinant replication-deficient adenovirus from bacterial β-galactosidase.