Fig. 3.
From: CD39 Modulates Endothelial Cell Activation and Apoptosis
CD39 decreases ATP-induced E-selectin mRNA levels.
Up-regulation of mRNA encoding for E-selectin was evaluated by Northern blots analysis in non-infected, β-galactosidase, or CD39 adenovirus-infected human umbilical vein endothelial cells (HUVEC) that were stimulated with thrombin, ATPγS, ATP, or adenosine (ADO) for 4 hr ATP-, but not ATPγS-induced E-selectin gene up-regulation was abrogated by CD39. Results shown are representative of three independent experiments and include the control glyceraldehyde-3-phosphate dehydrogenase (GAPDH). C, control; NI, non-infected; rAdCD39, recombinant replication-deficient adenovirus from human CD39 cDNA; rAdβgal, recombinant replication-deficient adenovirus from bacterial β-galactosidase.