Fig. 5
From: CD39 Modulates Endothelial Cell Activation and Apoptosis
Overexpression of CD39 inhibits ATP-induced apoptosis.
Confluent human umbilical vein endothelial cells (HUVEC) were left untreated or infected with CD39 or β-galactosidase adenovirus. Groups of cells were then left untreated or incubated with staurosporine (200 nM), ATP (5 mM), or ATPγS (5 mM). Cell apoptosis rates were assessed by flow cytometric analysis of DNA content by propidium iodide (PI) staining. ATP-induced apoptosis was decreased in rAdCD39-infected cells. FL2-A, forward light scatter; M1, viable cells; M2, nonviable cells.