From: Detection of Novel LAMC2 Mutations in Herlitz Junctional Epidermolysis Bullosa
Exon No. | 5′-primer (L)a | 3′-primer (R)a | Annealing Temp. (°C) | Product Size (bp) |
---|---|---|---|---|
1 | ggctctcttcctgtcaggac -70 | gaggtacgttcagccatcac +85 | 60 | 324 |
2 | gacgatctcttttgtatgct -30 | gttgttcccttccttcttct +96 | 55 | 315 |
3 | gcttctaatgccgcatacat -69 | ctcaaagcagtgtttggcac +114 | 60 | 319 |
4 | gttgtgaagcatttggaagc -52 | ctagttgggcaagggactct +54 | 55 | 205 |
5 | cctgatttctacaatgccac −25 | caaagccctgtacaacatta +53 | 55 | 215 |
6 | atagatgatgagggcttgtc −50 | tcctagtcctataagctggc +49 | 55 | 222 |
7 | tcatagcccaaagtgtcata −177 | aggcttgttgaacaaatgaa +102 | 60 | 469 |
8 | ggtatgtggtaagaatcgga −51 | ctacttgtttggagactgcc +93 | 55 | 255 |
9 | ctttagactgtaccacttgc −48 | gtctttcttacacagctctc +71 | 60 | 338 |
10 | attgtctgaccctgctcact −52 | cagagcagacccatggcctt +22 | 60 | 257 |
11 | cctaggcttggtcatttgtt −30 | aagtgcatacgtacacaggc +56 | 60 | 332 |
12 | aaggattctctgctgttgga −106 | acagaacacccagtggacac +72 | 60 | 321 |
13 | gtattggatgatcccttggg −65 | cctaacgtagccattccttc +68 | 55 | 290 |
14 | ttgctaactctatgctgacc −60 | tctacaagtaggtctcccaa +61 | 55 | 326 |
15 | aatggtgaatagaggcatgg −134 | aaactggtttcaaggtaggg +148 | 60 | 362 |
16 | ctaaatcagaccagctcccg −104 | ttacaactagcatggtgccc +95 | 60 | 357 |
17 | agtcctctttttattaccgc −43 | agaaagtatttatggccctc +54 | 55 | 242 |
18 | ggtgtggtaactggtaagca −54 | tattcatatggtgggcattc +60 | 55 | 267 |
19 | gtcagagtgattttgccagc −64 | caaaggaaattggtcatgac +66 | 55 | 245 |
20 | tttgctttcatcttcacggc −44 | tttccctccacaccaccctt +65 | 55 | 299 |
21 | ctatggttgtctttgggatg −76 | ataagcacaaaccagccttg +83 | 55 | 318 |
22 | gggagatccctttcaacttg −71 | tgagtgttctggagcaaagc +65 | 55 | 236 |
23 | agttatgggtatagaagggc −86 | tgacctgagcatacccatta 3821 in cDNA | 55 | 462 |