IC50 (ng/ml) Mean ± S.D.a
1.7 ± 0.5
6.2 ± 3.5
42.5 ± 17.5
64.5 ± 6.0
Normal endothelial cells HUAEC
- For cytotoxic assay, 104 cells/well were cultured in 96-well microtiter plates with different concentrations (0.1 to 1000 ng/ml) of IL-4(38-37)-pe38kdel, as detailed in Materials and Methods.
- aIC50, the concentration of IL-4 toxin at which 50% inhibition of protein synthesis occurred, was calculated from the graphs generated from the experimental data explained in Materials and Methods. The data are shown as the mean ± S.D. of two to five experiments done for each cell type.
- bIC50 was determined from a single experiment.
- cIC50 could not be achieved even at 1000 ng/ml concentration of IL-4 toxin.