Fig. 3
From: Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion
AQP1 mRNA levels in lamb tissues before and after CPB with HCA at 15°C
(A) Autoradiograph of RNAse protection assay. For each organ: left, 20 min sternotomy only; middle, CPB/HCA, no reperfusion; right, 6 hr of reperfusion following CPB/HCA. The expected AQP1-protected band is 125 nucleotides (nt) and the expected actin-protected band is 63 nt. Total RNA was lost from the lung control sample.
(B) Quantitation of AQP1 mRNA levels obtained from PhosphorImager analysis of the sequencing gel in A. Background was determined by tRNA control. AQP1 was normalized to the internal actin control, [AQP1-tRNA]/[actin-tRNA]. Negative numbers represent AQP1 signals weaker than background tRNA. Control, 20 min sternotomy only; no reperf, CPB/HCA without reperfusion; 6 hr reperf, 6 hr of reperfusion following CPB/HCA. Lung control data were not obtained in this experiment.