Fig. 6
From: Brain Expression of Presenilins in Sporadic and Early-onset, Familial Alzheimer’s Disease
Localization of presenilins within neurons containing neurofibrillary tangles in sporadic AD. Human Alzheimer’s disease (AD) brain sections were double-labeled with antisera to presenilin 2 (PS2) or presenilin 1 (PS1) and paired helical filament tau (PHF-tau) by the avidin-biotin (ABC) technique and the two chromogens, diaminobenzidine (DAB) and Vectastain SG (SG) and visualized by brightfield microscopy. Labeling with affinity-purified anti-PS2 antiserum (NT14; A and B) or (P1; C and D), both in brown, and the anti-PHF tau (AT-8), in blue (34), in neuronal pyramids of the entorhinal cortex (A and B) and hippocampus (C and D) and from sporadic AD brains demonstrate that a subpopulation of presenilin-positive neurons contain neurofibrillary pathology. The levels of presenilin immunolabeling in neurons containing neurofibrillary tangles, however, were similar to those in neurons not labeled by AT-8.