Table 1 Primers sequences used in this study
From: Involvement of the Endothelin System in Experimental Critical Hind Limb Ischemia
Product | Sense | Antisense | Size (bp) |
|---|---|---|---|
PreproET-1 | 5′-TCTCTGCTGTTTGTGGCTTTC-3′ | 5′-TCGGAGTTCTTTGTCTGTTTG-3′ | 368 |
PreproET-3 | 5′-GGAAATGCTGGGCAGGAG-3′ | 5′-CAGGTGCAGTTTCCGACTTC-3′ | 402 |
ECE-1 | 5′-CAAGCTCCTTTCTCGACCA-3′ | 5′-TTACCCAGTTCTGGTAGGCC-3′ | 856 |
ETA receptor | 5′-GGACAGGTACAGAGCAGTGG-3′ | 5′-CATTCATGGGGACCGAGG-3′ | 662 |
ETB receptor | 5′-ACAAGCTGCTGGCAGGGG-3′ | 5′-AACAGAGGGCAAACACGAGG-3′ | 426 |
GAPDH | 5′-GTGAAGGTCGGAGTCAACG-3′ | 5′-GGTGAAGACGCCAGTGGACTC-3′ | 299 |