Fig. 2

Induction of SMN and SMNc mRNAs in interferon (IFN)-treated A172 cells. Total RNA (1.5 µg) from A172 cells treated for the indicated time periods with medium alone (C) or in the presence of IFN-β (1000 U/ml) or IFN-γ (1000 U/ml) was reverse transcribed and polymerase chain reaction (PCR) coamplification was performed for SMN/SMNc and actin genes as described in “Materials and Methods.” The products were analyzed on a 2% agarose gel, visualized by ethidium bromide staining (upper panel), or previously subjected to digestion with Dra I and analyzed by 7% polyacrylamide gel electrophoresis (lower panel). The size of the PCR product for SMN, SMNc, and actin are, respectively, 126 bp, 102 bp, and 165 bp. Lane (0) represents the negative control, which was the product of PCR where RNA was omitted.