Fig. 6
From: Interferons and IRF-1 Induce Expression of the Survival Motor Neuron (SMN) Genes
Induction of SMN and SMNc gene expression in interferon (IFN)-treated fibroblasts. (A) Total protein extracts (100 µg/lane) from fibroblasts of control (C1, C2) and of type-I (SMA1, SMA2) or type-II (SMA3) Spinal muscular atrophy (SMA) patients were analyzed by Western blots analysis, as previously described in Fig.1 using the anti-SMN antiserum (1:1000). (B) Total RNA from control fibroblasts (C1) untreated or treated for 4 hr and 8 hr with IFN-β (1000 U/ml) or IFN-β (1000 U/ml) was reversed-transcribed and polymerase chain reaction (PCR) coamplification was performed for SMN/SMNc and actin genes, as described previously. The products were analyzed on a 2% agarose gel (upper panel) or previously digested with Dra I and analyzed by 7% polyacrylamide gel electrophoresis (lower panel). Lane (0) represents the negative control, which was the product of PCR where RNA was omitted.