Fig. 1
From: SM22α Promoter Targets Gene Expression to Vascular Smooth Muscle Cells In Vitro and In Vivo
Smooth muscle cell (SMC)-specific adenoviral vectors. (A) Structure of the adenoviral vectors, AdSM22α-hpAP and AdSM22α-CAT, encoding either human placental alkaline phosphatase (hpAP) or chloramphenicol transferase (CAT), respectively. Recombinant cytomegalovirus (CMV) adenoviral vectors drive reporter gene expression under control of the cytomegalovirus immediate early gene promoter/enhancer (CMV E/P) and/or intron (CMV IE 59UT). The adenoviral cosmid Sub360 genome has a single loxP sequence and deletion in the E1 and E3 regions (DE3), generating replication-defective adenovirus. The shuttle plasmid and adenoviral cosmid were recombined by Cre-loxP. (B) Southern blots analyses of AdSM22α-hpAP and AdSM22α-CAT. High molecular weight DNA was prepared from cells, and hybridized to the 32P-radiolabelled E1 cDNA probe under high stringency conditions. Respective shuttle plasmids and Ad5Sub360 adenoviral genomic cDNA served as negative and positive controls, respectively. Size markers (M) in kilobases are shown to the left of the blot.