Fig. 2
From: SM22α Promoter Targets Gene Expression to Vascular Smooth Muscle Cells In Vitro and In Vivo
Smooth muscle cell (SMC) specifity of AdSM22α-hpAP. Primary porcine aortic smcs (PASMC), jugular vein smcs (PJVSMC), mouse aortic smcs (MASMC), porcine aortic endothelial cells (PEC), and murine fibroblasts (NIH93T3) were infected with a viral titer of 250 or 500 (MOI) of AdSM22α-hpAP or AdCMV-hpAP. Two days after infection, cells were fixed and stained for alkaline phosphatase (AP) activity. Ectopic AP expression encoded by AdSM22α-hpAP was restricted to primary smcs and was not seen in endothelial cells or fibroblasts; whereas, the AdCMV-hpAP adenoviral vector programed AP expression in both smooth and non-smooth muscle cell lineages.