Fig. 4
From: SM22α Promoter Targets Gene Expression to Vascular Smooth Muscle Cells In Vitro and In Vivo
Alkaline phosphatase (AP) expression in injured and infected porcine femoral arteries. (A) Following infection with adenovirus AdSM22α-hpAP, only intimal and medial smooth muscle cells ectopically expressed AP (B and C). In contrast, in addition to vascular smooth muscle cells (smcs), luminal endothelial and adventitial cells expressed recombinant AP protein after infection AdCMV-hpAP (D and E). The sections were slightly counterstained by methyl green. Arrowhead indicates internal elastic lamina. Original magnification is 200X. hpAP, human placental alkaline phosphatase; CMV, cytomegalovirus. m 5 media, a 5 adventitia