Fig. 5
From: SM22α Promoter Targets Gene Expression to Vascular Smooth Muscle Cells In Vitro and In Vivo
Expression of alkaline phosphatase (AP) in vivo. Comparison of the number of intimal and medial AP positive cells between the femoral arteries infected either with adenoviruses AdSM22α-hpAP or AdCMV-hpAP four days after injury and infection in the pig. In each arterial segment, the number of AP-positive cells in the intima was counted in four randomly chosen areas; whereas, AP-positive medial cells were counted in the entire section. The total number of cells was obtained from the identical areas of hematoxylin and eosin (H&E) stained sections. Data is expressed as mean 6 standard error of the mean (SEM) values. The number of transduced cells was compared by analysis of variance. *p, 0.01. hpAP, human placental alkaline phosphatase; CMV, cytomegalovirus.