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Fig. 2 | Molecular Medicine

Fig. 2

From: Essential Components of Antimicrobial Gastrointestinal Epithelial Barrier: Specific Interaction of Mucin with an Integral Apical Membrane Protein of Gastric Mucosa

Fig. 2

Hydrolysis of mucin binding protein (MBP). (A) Nitrous acid hydrolysis and partition of rat mucosal MBP in Triton X-114 (22,23). Lane 1, Triton X-114 extract of MBP treated with nitrous acid (HONO). Lane 2, Triton X-114 extract of MBP treated with HONO2 (mock hydrolysis). Lanes 3 and 4, the aliquots of MBP before incubation in HONO, and HONO2, respectively. (B) Water phase from the MBP preparations treated with HONO, corresponding to lanes 1–3 in panel A. The protein transferred from SDS-PAGE to cellulose membrane was stained using Immun-Blot kit for glycoprotein detection (BioRad). The carbohydrate labeling with biotin was performed with protein immobilized in nitrocellulose.

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Molecular Medicine

ISSN: 1528-3658