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Fig. 4 | Molecular Medicine

Fig. 4

From: Essential Components of Antimicrobial Gastrointestinal Epithelial Barrier: Specific Interaction of Mucin with an Integral Apical Membrane Protein of Gastric Mucosa

Fig. 4

Mucin mucin binding protein (MBP) complexes from rat gastric mucosa. Gastric mucosal membranes prepared as described in (11) or by 1% Triton X-100 treatment (20) were subjected to Triton X-114 partition, SDS-PAGE, and immunoblotting. (A) Silver-stained SDS-PAGE gel of six separate preparations of MBP as in (11). Lane 7, purified rat gastric mucin (Muc). (B) Silver-stained SDS-PAGE gel of the samples obtained from the membranes isolated as in (11) or after 1% Triton extraction (20) and the transfer of MBP to nitrocellulose. Lane 1 depicts purified rat gastric mucin. Lanes 2–4 show the samples, as in the lanes 1–3, panel A. Lanes 5–7 depict material obtained from the mucosal membranes treated with 1% Triton X-100.

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Molecular Medicine

ISSN: 1528-3658