Fig. 4

Characterization of Vδ1 T cell phenotype and Vγ-rearrangements. (A) The expression of several differentiation markers on Vδ1 T cells was evaluated by flow cytometry after three-color labeling with anti-Vδ1, anti-CD62L, anti-CD45-RO, anti-HLA-DR, anti-CD95, anti-CD4, and anti-CD8 antibodies (mAbs). Results are expressed as arithmetic mean percentage ± standard deviation (SD) of positive cells among peripheral (white bars) and liver infiltrating (hatched bars) Vδ1⁺ T cell subset from 10 hepatitis C virus (HCV)-infected patients. (B) The T cell receptor (TCR) repertoire of peripheral (white bars) and intrahepatic (hatched bars) Vδ1+ T cells was analyzed by flow cytometry using specific mAbs directed against Vγ regions (Vγ2-3-4, Vγ3-5, Vγ8, Vγ9) on six HCV-infected patients. Significance refers to Student’s t-test. p-values are indicated as follows: * = p < 0.05. PBMC, peripheral blood mononuclear cells; intrahepatic lymphocytes (IHL).