Fig. 2

The effects of adriamycin on KLE cells. KLE cells (100 nM and 5 µM; final concentration) were cultured with Dulbecco’s modified Eagle’s medium/F-12 (DMEM/F-12), containing 0.5% calf serum (CS), 2.5% CS and 5% CS, as described in “Materials and Methods. (Upper panel) The effects of adriamycin on the expected number of KLE cells. Note the dramatic decrease in the number of expected KLE cells in culture after 48 hr incubation with adriamycin (100 nM and 5 µM). Expected number of the KLE cells was determined in adriamycin-free (control) cell cultures. Increasing concentration of CS did not alter the adriamycin effects on KLE cells; whereas, 5% CS provided the optimum milieu for the growth of KLE cells in adriamycin-free cultures. (Lower panel) The dose-dependent increase of the number of dead KLE cells after 48 hr exposure to adriamycin (100 nM and 5 µM) in media containing 5% CS, determined by trypan blue exclusion.