Fig. 4
From: Increased Insulin-like Growth Factor 1 Activity Can Rescue KLE Endometrial-like Cells from Apoptosis
Adriamycin (100 nM) induced the apoptosis of KLE cells. This was confirmed by the analysis of DNA fragmentation on simple agarose gel. Note the classic DNA ladders (multiple of 180 bp fragments) that accompany programmed cell death in vitro. KLE cells were growing in Dulbecco’s modified Eagle’s medium/F-12 (DMEM/F-12) containing 5% calf serum (CS) and apoptosis was triggered by the exposure to 100 nM of adriamycin for 48 hr, as described in “Materials and Methods.”