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Fig. 1 | Molecular Medicine

Fig. 1

From: Purification and Characterization of Macrophage Migration Inhibitory Factor as a Secretory Protein from Rat Epididymis: Evidences for Alternative Release and Transfer to Spermatozoa

Fig. 1

Western blots analysis comparing rat testicular cytosol, epididymal cytosol (caput, corpus, cauda region), isolated epididymal fluid (caput, corpus, cauda region), and vesicles isolated from epididymal fluid (25 µg per lane). Specimens were probed with the antibody directed against migration inhibitory factor (MIF). The immunoreactive band at 12 kDa was comigrating with recombinant MIF (rec MIF, 2 µg). Highest amounts were detected in the epididymal cytosol and fluid fractions, with much lower quantities found in the corpus and cauda samples. The clear MIF band was also present in isolated vesicles from epididymal fluid.

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Molecular Medicine

ISSN: 1528-3658