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Fig. 2 | Molecular Medicine

Fig. 2

From: Purification and Characterization of Macrophage Migration Inhibitory Factor as a Secretory Protein from Rat Epididymis: Evidences for Alternative Release and Transfer to Spermatozoa

Fig. 2

Immunohistochemistry (A-C) and in situ hybridization (D-F) of MIF in rat epididymis. (A) Caput epididymis. Strong migration inhibitory factor (MIF) immunoreaction is visible in the epithelial cells (arrows); whereas, the stroma is negative. (B) In the cauda region, MIF labeling in epithelial cells is clearly less intense. Note the predominant apical staining of the epithelium (arrowheads). (C) Negative control using anti-MIF antibody preabsorbed with recombinant MIF (rec MIF). (D) Caput region. In situ hybridization shows strong MIF mRNA expression in the epithelial cells (arrows). Again, the stroma is negative. (E) Corresponding to the immunohistochemical results, the cauda region shows substantially weaker MIF expression. (F) Sense negative control, caput region. Bar = 40 µm.

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Molecular Medicine

ISSN: 1528-3658