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Fig. 4 | Molecular Medicine

Fig. 4

From: Purification and Characterization of Macrophage Migration Inhibitory Factor as a Secretory Protein from Rat Epididymis: Evidences for Alternative Release and Transfer to Spermatozoa

Fig. 4

Laser scanning micrograph of MIF-immunofluorescence labeling in isolated epididymal spermatozoa. Several spermatozoa are visible. Triton X-100 pretreatment was required to obtain a signal. Migration inhibitory factor (MIF) is localized in the sperm midpiece (arrows) and principal piece (arrowheads); whereas, the sperm heads (asterix) and the distal end of the sperm tail are clearly negative. The MIF distribution correlates with the localization of the outer dense fibers (ODF) in spermatozoa. Bar = 10 µm.

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Molecular Medicine

ISSN: 1528-3658