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Fig. 5 | Molecular Medicine

Fig. 5

From: Purification and Characterization of Macrophage Migration Inhibitory Factor as a Secretory Protein from Rat Epididymis: Evidences for Alternative Release and Transfer to Spermatozoa

Fig. 5

Expression of MIF mRNA in the rat epididymis. (A) Northern blots analysis using a digoxigenin-labeled cRNA probe derived from the reverse transcription-polymerase chain reaction (RT-PCR) fragment. Migration inhibitory factor (MIF) mRNA shows a spatial expression pattern, with the highest amounts in the caput region. (B): The respective RNA gel stained with ethidium bromide is serving as a loading control. Lane 1: rat testis; lane 2: rat epididymis caput region; lane 3: epididymis corpus region; lane 4: epididymis cauda region.

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