Fig. 8

Reduction of cell death by the proteasome inhibitors. Neurons were treated with colchicine (1 µM) and N-benzyloxycarbonyl-Val-Ala-aspartyl-fluoromethyl ketone (zVAD-fmk; 100 µM). After 12 hr incubation, solvent control dimethylsulfoxide (DMSO), additional zVAD-fmk (100 µM), lactacystin (20 µM), or N-benzyloxycarbonyl-Leu-Leu-Nva-aldehyde (MG 115; 5 µM) were added for a further incubation time of 18 hr. After 30 hr, neurons were stained with H-33342 and cell death is expressed as percentage of nuclei with condensed chromatin. Data are mean values from triplicate determinations. * p < 0.05; solvent vs. proteasome inhibitors. SD, standard deviation.