Fig. 3
From: p27Kip1 Localizes to Detergent-insoluble Microdomains Within Lymphocyte Membranes
p27 expression in detergent-treated lymphocytes. Freshly harvested peripheral blood lymphocytes were incubated in cold buffer containing 0.5% NP-40 (A) or buffer lacking NP-40 (B) for 30 min, washed, fixed, permeabilized, and stained in suspension for p27 expression. Confocal images acquired at equal (pmt) sensitivity are shown. Membrane, but not nuclear expression of p27, persists after NP-40.