Fig. 6
From: p27Kip1 Localizes to Detergent-insoluble Microdomains Within Lymphocyte Membranes
Patching of CD69, but not of p27, upon mitogen stimulation of lymphocytes. Lymphocytes were incubated for 16 hr in the presence of medium alone (unactivated) or medium containing phorbol ester 12-O-tetradecanoylphorbol-13-acetate (PMA; 100 ng) and phytohemagglutinin (PHA; 1 µg/ml), and stained for the lipid analogue DIOC16(3) (green), p27 (blue) or CD69 (red), as described in the text. Areas of overlap of DIOC16(3) and p27 appear as white (overlay in 4th column); overlap of CD69 and DIOC16 (3) are yellow.