Fig. 3
From: A Therapeutic Target for Hormone-independent Estrogen Receptor-positive Breast Cancers
Sensitivity of the growth of 21PT and MCF-7 cells to (A) tamoxifen (TAM) and (B) W7. MDA-MB231, 21PT, and MCF-7 cells (2 × 104) were plated in duplicate in 96-well tissue culture dishes in 0.3 ml of rich medium and grown under standard tissue culture conditions. 24 hr later, medium was removed and fresh medium, plus drugs, was added to achieve the indicated concentrations. The cells were grown for an additional 5 days. Cells in control wells were treated with equivalent amounts of solvent (dimethylsulfoxide, DMSO). Medium was changed on alternate days and replaced with fresh medium and drugs. Cell viability in the presence of the drugs was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay (31) and expressed as percentage of the untreated control. The experiment was repeated three times.