Fig. 2

Nucleotide sequence of the upstream promoter region (exon 1a) of the human liver/bone/kidney alkaline phosphatase (L/B/K ALP) gene. The numbers on the right side of the nucleotide sequence refer to European Molecular Biology Laboratory (EMBL) accession numbers M24428 (1–806) and M24429 (1–119); whereas, those on the left side are as described (38). The major transcription start sites are shown as black squares. Black dots indicate the minor starts of transcription. The position of the first base of the ATG translation initiation codon (exon 2) is indicated as +1. The arrows mark the 672-bp Ava II restriction fragment bearing the promoter activity (38). The gray box indicates the nucleotide sequence used as probe for the experiments described in Figs. 3–5. In the boxed region, the atypical 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-response element (TRE) is highlighted in bold. Putative binding sites for the transcription factors Sp1; (underlined), upstream stimulatory factor (USF) (overlined), and GATA (broken line) present in the Ava II fragment are also shown. The computer program MatInspector (68) was used to identify the core consensus sequences described. Modified from Weiss et al. (38).