Fig. 1

Immunoreactivity of the selected phages with patients and controls. (A) Reactivity of breast cancer patient sera and controls to random phages derived from the 6th round of selection. Following transfer to nitrocellulose and blocking, the membranes were incubated with (8 µg/mL) pooled IgG fraction from breast cancer patients (b), lymphoma (l), osteosarcoma (o), or normal (n). The use of alkaline phosphatase conjugated anti-human IgG antibodies as described in Materials and Methods detected the immunoreactive phage clones. (B) Reactivity of phage particles with individual serum as detected by immunospot assays. In these experiments purified phage particles (10⁶ TU/spot) were spotted onto nitrocellulose, blocked and then processed as in A. Each serum was used at 1/500 dilution. F1 and F8 are patients with breast cancer. N7 and N9 are normal individuals.