Fig. 3
From: Antigen-Specific IgG Antibodies in Stage IV Long-Time Survival Breast Cancer Patients
Western blot analysis of cellular protein extracts from breast cancer cell line PMI. Extracts were separated by 10% polyacrylamide gel electrophoresis, transferred to nitrocellulose, and incubated with serum from 3 patients with breast cancer who strongly responded to the SQRIPARIHHFPTSI peptide (A, lanes 1–3), 3 patients with breast cancer who did not respond to the peptide (B, lanes 1–3), 3 normal individuals (C, lanes 1–3), 5 patients with prostate cancer (D, lanes 1–5) and 2 patients with osteosarcoma (E, lanes 1 and 2). All sera were used at dilution 1/1000. (F) Western blots of cell extracts prepared from peripheral blood mononuclear cells (lane 1) and from the breast cancer cell line PMI (lane 2). Sera from patient 3 (A) was used. In D sera from patient 3 was used as positive control (C).