Fig. 3

Rabbit EBA-175 RII specific antibodies block native EBA-175 binding to human erythrocytes. [³⁵S]-labeled EBA-175 culture supernatants were incubated with a titration of RII specific antibodies and then added to human erythrocytes. Erythrocytes were pelleted as described in Materials and Methods and bound [³⁵S]-labeled EBA-175 was eluted and detected by phospho-imaging (A). B shows the percent blocking of native EBA-175 binding to erythrocytes as compared to the control sera at a 1/10 dilution.