Fig. 4
From: Melanoma Differentiation Associated Gene-7 (mda-7): A Novel Anti-Tumor Gene for Cancer Gene Therapy
Ad-mda7-induced rapid apoptosis and cell cycle regulation. Panels A and B shows rapid induction of apoptosis in H1299 and DLD-1 cells infected with Ad-mda7 or Ad-luc, respectively. Cells were evaluated at different time points post-infection for Annexin V-FITC staining by FACS analysis. Panel C shows the cell cycle analysis of Ad-mda7 or Ad-luc transduced T47D breast cancer cells. A dose-dependent increase in G2/M-block and apoptosis (Sub G0/G1) is observed. Increasing MOI (vp/cell) of Ad-mda7 and Ad-luc (control) are denoted as M0, M500, M1000, M2000 and L0, L500, L1000, L2000, respectively.