Fig. 2

Biological effects of the L. sativus seed IPG. (A) The ability of IPG and rIPG to inhibit the activity of PKA in an in vitro assay. ^*Taken from Villalba et al. (21). (B) The effect of no additions, IPG (10 µM), rIPG (10 µM) or IGF-I (1 nM) on the growth of organotypic cultures of CVG. The calibration bar represents 100 µm. The numbers below each photograph represent CVG volume measurements (arbitrary units). (C) Upper: Western blot detection of PCNA expression within the CVG after treatment with no additions, IPG (10 µM), rIPG (10 µM) and IGF-I (1 nM). Lower: Densitometric analysis of the band corresponding to PCNA. IPG was obtained by hydrolysis of L. sativus seed GPI with GPI-PLD.