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Table 2 Hydrolysis of L. sativus seed GPI by phospholipases C and D.

From: Purification and Characterization of Insulin-Mimetic Inositol Phosphoglycan-Like Molecules From Grass Pea (Lathyrus sativus) Seeds

 

Enzyme

 

GPI-PLD

PI-PLC

GPI-PLC

Liposomal Composition

Lag time (min)

Rate (nmol GPI/min)

Hydrolysis

(%GPI) 10 min

Hydrolysis

(%GPI) 15 min

Hydrolysis

(%GPI) 60 min

Hydrolysis

(%GPI) 60 min

Hydrolysis

(%GPI) 60 min

GPI/PtdCho/PtdEth

       

(1:1:2)

0

0.092

13.2

14.8

   

0.07mMa

       

0.08mMa

    

20

  

GPI/PtdEth/PtdCho/Ch

       

(10%:2:1:1)

       

0.33mMa

    

32

21

14

GPI/PtdEth/PtdCho/Ch

       

(8:46:23:23)

       

0.33mMa

 

0.53

   

0.35

0.23

  1. L. sativus seed GPI was reconstituted in liposomal membranes and then incubated with either GPI-PLD, GPI-PLC, or PI-PLC.
  2. aAll liposome preparations contained 13 µg GPI at the start. The SEM showed <10% variation.
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Molecular Medicine

ISSN: 1528-3658