Skip to main content
Fig. 3 | Molecular Medicine

Fig. 3

From: Species-Crossreactive scFv Against the Tumor Stroma Marker “Fibroblast Activation Protein” Selected by Phage Display From an Immunized FAP−/− Knock-Out Mouse

Fig. 3

Sandwich ELISA for affinity determination of scFv MO36 (A) and scFv MO33 (B). Bacterial lysates of scFv MO36 and partially purified scFv MO33 were diluted and incubated on human or murine CD8-FAP fusion protein immobilized via a CD8-specific rat mab. For detection, biotinylated c-myc-specific mab 9E10, POD-streptavidin, and ABTS substrate (measured at 405 nm) were used.

Back to article page

Molecular Medicine

ISSN: 1528-3658