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Fig. 5 | Molecular Medicine

Fig. 5

From: Species-Crossreactive scFv Against the Tumor Stroma Marker “Fibroblast Activation Protein” Selected by Phage Display From an Immunized FAP−/− Knock-Out Mouse

Fig. 5

Competition of scFv MO33 and scFv MO36 by the chimeric mab cF19γ1. Diluted bacterial lysate (1:50) of the scFv were mixed with variable concentrations of cF19γ1. The mixtures were incubated on CD8-hFAP in an ELISA. One hundred percent competition reflects a reduction of specific ELISA signal to background level (OD405 nm ≈ 0.03) whereas 0% competition reflects the ELISA signal without competitor (OD405 nm ≈1).

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Molecular Medicine

ISSN: 1528-3658