Fig. 1

A representative differential screening dot blot of subtractive hybridization clones. Dot blot analysis was completed on clones isolated from subtractive hybridization experiments. Blots were probed with the subtracted library “positive” and the reverse subtracted library “negative” (10⁶ cpm per 100 ng of subtracted cDNA probe) in ExpressHyb overnight at 65°C. After washing, the membranes were autoradiographed overnight at −70°C. The positions of the clones are marked by numbers 1–10 and letters A–G. Clones identified through sequence analysis to encode portions of the keratocan gene that are up-regulated in the “positive” subtracted library (compared to the “negative”) are depicted by the arrows.