Fig. 4
From: Keratocan Expression Is Increased in the Stroma of Keratoconus Corneas
In situ hybridization using a keratocan cDNA probe. Corneal sections were from a 15-month-old normal donors (A–C), two patients with keratoconus (D and E, 22-year-old; F, 17-year-old), two patients with Fuchs’ corneal dystrophy (G, 54-year-old; H, 52-year-old), and a 77-year-old patient with aphakic bullous keratopathy (I). Hybridization without probe served as a negative control in B. Positive hybridization products appear as blue deposits. Note the increase in staining in keratocytes (D–F) of keratoconus specimens compared to those of normal (A and C) and other diseased (G–I) corneas. *corneal epithelium; St, stroma. (Original magnification, ×10 for A, B and D; ×40 for C and E–I).