Fig. 5
From: Keratocan Expression Is Increased in the Stroma of Keratoconus Corneas
Relative quantitative RT-PCR of keratocan. Total RNA samples were from 20-, 22-, 35-, 45-, and 54-year-old normal human (NH) donors, 25-, 34-, 36-, 45-, and 62-year-old patients with keratoconus (KC), 51- and 80-year-old patients with Fuchs’ corneal dystrophy (FUCHS’), 68- and 86-year-old patients with pseudophakic bullous keratopathy (PBK), and a 36-year-old patient with a corneal scar (SC). The keratocan and 18S rRNA bands are labeled. Total RNA was used as template instead of cDNA as a negative control for relative RT-PCR (lane not shown). No products were detected in the negative control.