Fig. 3
From: Macrophage-Derived Heme-Oxygenase-1: Expression, Regulation, and Possible Functions in Skin Repair
HO-1 expression is predominantly restricted to wound macrophages. Frozen serial 6-µm sections from 5-day mouse wounds were incubated with a polyclonal antibody directed against human HO-1 (A, C, E, F), or a monoclonal antibody directed against the murine macrophage-specific F4/80 antigen (B, D). Note that A and B, or C and D represent directly neighbored serial sections. All sections were stained with the avidin-biotin-peroxidase complex system using 3–3 diaminobenzidine-tetra-hydrochloride as a chromogenic substrate. Nuclei were counterstained with hematoxylin. Strongly immunopositive signals within the sections are indicated with arrows. Scale bars are 200 µm for A and B, 100 µm for C, D, E, and 50 µm for F. G, granulation tissue; HE, hyperproliferative epithelium; HF, hair follicle; M, muscle; S, scab.