Fig. 5
From: Macrophage-Derived Heme-Oxygenase-1: Expression, Regulation, and Possible Functions in Skin Repair
NO does not influence HO-1 expression during skin repair in vivo. Total cellular RNA (15 µg) from non-wounded and wounded back skin of PBS (−) and L-NIL (+) injected mice of two independent experimental series (#I, #II as indicated) was analyzed by RNase protection assay for the presence of HO-1 mRNA. Sixteen wounds from the backs of four animals were excised for each experimental time point and used for RNA isolation. The time after injury is indicated at the top of each lane. “Control skin” refers to nonwounded skin. One thousand cpm of the hybridization probe were added to the lane labeled “probe.”